Epimedium extract icariin

Epimedium extract icariin Analytical method

Epimedium extract icariin Thin layer identification method

 Take 0.5g of epimedium medicinal powder or an appropriate amount of extract, add 10ml of ethanol, soak in warm temperature for 30 minutes, filter, evaporate the filtrate to dryness, add 1ml of ethanol to the residue to dissolve, which is the test solution. Weigh the icariin reference substance, dissolve it in methanol and dilute it to 0.1 mg/ml, which is the reference substance solution. Take 10 μl each of the test solution and the reference solution, and place them on the same silica gel H thin-layer plate with sodium carboxymethylcellulose as the adhesive, and use ethyl acetate-butanone-formic acid-water (10:1: 1:1) is the developing agent, unfold it, take it out, dry it, and inspect it under a UV lamp (365nm). In the chromatogram of the test product, the same dark red spots appear at the positions corresponding to the chromatogram of the reference substance; spray with trichloride Aluminum test solution, and then placed under UV light (365nm) for inspection, the same orange-red fluorescent spots were revealed.

Epimedium extract icariin determination of total flavonoid content in Epimedium and its extracts by spectrophotometry

 Precisely weigh the reference substance icariin and prepare a standard solution of 10ug/ml with methanol. Precisely weigh an appropriate amount of the powder of this product (equivalent to approximately 1 mg of icariin), ultrasonic with 25 ml of methanol for 30 minutes, dilute to volume in a 50 ml volumetric flask, and filter with a 0.45 μm microporous membrane to prepare for the test. product solution. Take the test solution and the reference solution respectively, use the reagent as the blank, measure the absorbance at a wavelength of 270nm, and calculate it.

Epimedium extract icariin determination of icariin content in Epimedium and its extracts by HPLC method

 Chromatographic conditions and system suitability test: ODS25uC18 (4mm×150mm) chromatographic column; mobile phase: acetonitrile-water (30:70); detection wavelength: 270nm; flow rate: 0.8ml/min. The number of theoretical plates based on icariin is not less than 1500. Preparation of the reference substance solution: Precisely weigh the reference substance icariin and prepare a 0.1 mg/ml solution with methanol. Preparation of the test solution: Precisely weigh an appropriate amount of this product powder (equivalent to about 1 mg of icariin), sonicate with 25 ml of methanol for 30 minutes, dilute to volume in a 50 ml measuring bottle, filter with a 0.45 μm micropore Membrane filtration is the test solution. Determination method: Inject 10 μl of each sample test solution and reference solution into the HPLC instrument, and calculate the area using the external standard method.

Epimedium extract icariin pharmacological effects

 1. Effect on sexual function Epimedium extract has a certain effect on promoting gonadal function. Flavonoids such as icariin have the effect of tonifying the kidneys and strengthening yang. 30-1000 μg/l can promote the secretion of estradiol by follicular granulosa cells, and 1000 μg/l can promote the secretion of corticosterone by adrenocortical cells. At the same time, EPS can increase gonadal hormone levels, mainly increasing FSH levels.

 2. Improve the body’s immune function. 50% methanol extract of Epimedium can inhibit lymphocyte transformation. Epimedium total flavonoids can increase cell function and antibodies in mice with “Yang deficiency”, and significantly enhance human T cell immune function.  Both EPS and EI have immunomodulatory effects.

 3. Antioxidant EPS and EI both have antioxidant activity, which can enhance antioxidant enzyme activity and reduce the effects of free radical products.

 4. Anti-aging effect: Epimedium extract can resist aging and prolong life by affecting cell passage, prolonging the growth period, regulating the immune and secretory systems, and improving body metabolism and the functions of various organs.

 5. Cardiovascular effects: The non-amino acid part of Chemicalbook in the icariol extract can significantly increase the coronary flow of isolated rabbit hearts. Decoctions and alcohol infusions have a blood pressure lowering effect. Icariin can directly relax vascular smooth muscles and dilate coronary arteries, femoral arteries and cerebral arteries. The mechanism of action is to inhibit the influx of extracellular calcium ions into vascular smooth muscle.

 6. Anti-inflammatory and anti-allergic effects Epimedium methanol extract can significantly reduce the swelling of egg white “arthritis” in rats and reduce the increase in capillary permeability of rabbits caused by histamine. It can also inhibit allergic asthma in guinea pigs caused by histamine and acetylcholine.

 7. Effect on bone growth. Epimedium extract has the activity of inhibiting osteoclasts, while promoting the function of osteoblasts, increasing the formation of calcified bone, and promoting the synthesis of DNA in bone marrow cells, which can not only prevent the occurrence of castration. It can prevent osteoporosis caused by rats and can also prevent osteopenia and osteoporosis caused by hormones. Other studies have shown that epimedium injection has a significant promoting effect on the growth of chicken embryo femurs in vitro.

 8. Other effects: Epimedium crude extract has expectorant, antitussive and antiasthmatic effects.

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